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Healing / RecoveryInjectableResearch Protocol

TB-500 (Thymosin Beta-4) Dosage Chart

TB-500 is a synthetic version of the active region of Thymosin Beta-4, an actin-binding peptide studied for cell migration, angiogenesis, and wound, tendon, and muscle repair. Research protocols run a loading phase then a lower maintenance phase, and it is most often paired with BPC-157 for recovery work. Nearly all efficacy data is preclinical.

AI-built tool. Cross-check critical values against authoritative sources before use.

Half-life

Short plasma presence; sustained tissue activity (dosed 1-2x weekly)

Format

Injectable

Purity

≥98%

Protocols

3 variants

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Research Protocols

Dosage Protocols

Each row reflects a common research protocol variant. These are reference ranges derived from published literature and community research — not medical guidance.

Protocol 1

Loading Protocol

4-6 weeks

Dose

2-2.5mg subcutaneous, twice weekly

Duration

4-6 weeks

The initial loading phase most research protocols use to reach tissue saturation. Split across two weekly injections. Injected subcutaneously, often near the area of interest for localized research.

Protocol 2

Maintenance Protocol

Ongoing, as studied

Dose

2-2.5mg subcutaneous, once weekly (or every other week)

Duration

Ongoing, as studied

After the loading block, the frequency drops. TB-500's sustained tissue activity is why weekly or biweekly dosing is used rather than daily.

Protocol 3

Recovery Stack (with BPC-157)

Matched to the TB-500 block

Dose

TB-500 (above) + BPC-157 250-500mcg daily

Duration

Matched to the TB-500 block

The classic 'Wolverine' recovery pairing. TB-500 drives cell migration systemically while BPC-157 acts locally on angiogenesis and gut/tendon repair. See the BPC-157 chart for its dosing.

Mixing Math

Reconstitution

Reference values for preparing a research vial. Adjust the syringe reading if you change the water volume.

Vial size

10mg vial

BAC water

2ml bacteriostatic water

Concentration

5mg/ml

Dose / ml

2.5mg = 0.5ml

Syringe units

50 IU per 2.5mg on a U-100 syringe

Need help converting different vial sizes? Use our reconstitution calculator to generate protocol-specific numbers instantly.

Storage

Handling Notes

Lyophilized: -20°C long-term, 2-8°C short-term. Reconstituted with bacteriostatic water: refrigerate at 2-8°C and use within about 21-28 days. Swirl gently, never shake, and do not freeze the reconstituted solution.

Research Considerations

Cautions

  • Nearly all TB-500 healing evidence is preclinical (animal and cell studies); human efficacy is not established.
  • The same pro-angiogenic action that aids repair is a theoretical concern in anyone with an active or recent malignancy.
  • TB-500 is prohibited in sport under WADA (S2). Tested athletes must avoid it entirely; there is no therapeutic-use exemption route for a non-approved compound.
  • TB-500 is a research compound, not an approved therapy. Source only COA-verified material, since grey-market purity varies sharply.

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Research use only. Not medical advice. Browse all dosage charts