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LongevityInjectableResearch Protocol

NAD+ Dosage Chart

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to cellular energy metabolism, DNA repair, and sirtuin activity. Research and clinic protocols explore subcutaneous micro-dosing and slow intravenous administration for metabolic and longevity contexts.

AI-built tool. Cross-check critical values against authoritative sources before use.

Half-life

Variable (rapidly metabolised; delivered as a bolus or drip)

Format

Injectable

Purity

≥98%

Protocols

3 variants

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Research Protocols

Dosage Protocols

Each row reflects a common research protocol variant. These are reference ranges derived from published literature and community research — not medical guidance.

Protocol 1

Subcutaneous Micro-Dose Protocol

2-4 weeks, then reassess

Dose

50-100mg once daily

Duration

2-4 weeks, then reassess

Small subcutaneous doses are used because NAD+ injection can sting and cause a flushing sensation. Inject slowly. Start at the low end.

Protocol 2

Intravenous Drip Protocol

1-2 times per week

Dose

250-500mg as a slow IV infusion

Duration

1-2 times per week

IV NAD+ must be infused slowly (often over 1-2+ hours); rapid administration causes chest tightness, nausea, and flushing. Clinical-setting protocol only.

Protocol 3

Maintenance Protocol

Ongoing (cycled)

Dose

50mg subcutaneous 2-3 times per week

Duration

Ongoing (cycled)

Lower-frequency subcutaneous dosing after an initial phase. Often paired with NAD+ precursors like NMN in longevity research.

Mixing Math

Reconstitution

Reference values for preparing a research vial. Adjust the syringe reading if you change the water volume.

Vial size

1000mg vial

BAC water

5ml bacteriostatic water

Concentration

200mg/ml

Dose / ml

50mg = 0.25ml · 100mg = 0.5ml

Syringe units

25 IU (50mg) · 50 IU (100mg) on a U-100 syringe

Need help converting different vial sizes? Use our reconstitution calculator to generate protocol-specific numbers instantly.

Storage

Handling Notes

Lyophilized: 2-8°C preferred, -20°C for extended storage. Reconstituted: refrigerate at 2-8°C and use within about 28 days. Protect from light. NAD+ solutions degrade faster than many peptides; prepare conservatively.

Research Considerations

Cautions

  • Rapid administration is the main issue: inject subcutaneous doses slowly and infuse IV over an extended period to avoid chest tightness, nausea, and flushing.
  • NAD+ is a research compound in injectable form and is not an approved drug; source only COA-verified material.
  • Benefits for longevity endpoints in humans remain largely unproven; most evidence is mechanistic or preclinical.

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Research use only. Not medical advice. Browse all dosage charts