GLOW Peptide Dosage Chart
GLOW peptide is the common research name for a premixed 70mg vial: GHK-Cu 50mg, BPC-157 10mg, and TB-500 10mg. There is no published three-compound trial. This chart sizes a daily subcutaneous draw to the GHK-Cu, then shows the BPC-157 and TB-500 that ride along at that 5:1:1 ratio.
Half-life
Multi-compound (see individual peptides)
Format
Injectable
Purity
≥98%
Protocols
3 variants
Research Protocols
Dosage Protocols
No GLOW blend has been tested as a three-peptide combination in a peer-reviewed trial. The rows below are a conservative community protocol for the usual 70mg 50/10/10 vial sold by research vendors, including the Ascension GLOW 70mg SKU. Dose the GHK-Cu. Then read off what BPC-157 and TB-500 that draw actually delivers. If your label is not 50/10/10, redo the math before you copy these units.
Protocol 1
Standard 50/10/10 Daily
Dose
GHK-Cu 2mg + BPC-157 400mcg + TB-500 400mcg daily
Duration
4-6 weeks
One subcutaneous draw sized to 2mg GHK-Cu on a 50/10/10 vial. After 3ml bacteriostatic water that is 12 IU on a U-100 syringe. Matches standalone GHK-Cu research doses (1-2mg SC) and keeps BPC-157 inside the common 250-500mcg daily range. TB-500 at 400mcg daily is a blended-vial microdose, not the 2-2.5mg twice-weekly standalone loading protocol.
Protocol 2
Conservative / Skin-Focused
Dose
GHK-Cu 1mg + BPC-157 200mcg + TB-500 200mcg daily
Duration
6-8 weeks
Half-draw (6 IU after 3ml BAC). Used when GHK-Cu injection sting is the limiter, or when the research window is longer and lower copper exposure is the point. You cannot raise BPC-157 or TB-500 on this vial without also raising GHK-Cu.
Protocol 3
Recovery Add-On (separate vials)
Dose
Keep GLOW at the standard draw; add standalone BPC-157 or TB-500
Duration
Match the GLOW cycle
A premixed vial has a fixed ratio. You cannot raise BPC-157 or TB-500 from 50/10/10 without also raising GHK-Cu. If connective-tissue work needs more BPC-157 or a true TB-500 load without more copper, add those from separate vials or use the Wolverine stack alongside a lower GLOW draw.
Week by Week
GLOW Peptide Week-by-Week Protocol
| Week | Dose | Notes |
|---|---|---|
| Week 1 | GHK-Cu 1mg + BPC-157 200mcg + TB-500 200mcg | Half-draw (6 IU after 3ml BAC). Community start used to check GHK-Cu injection sting and local reaction before the full daily draw. Not a published titration rung. |
| Weeks 2-5 | GHK-Cu 2mg + BPC-157 400mcg + TB-500 400mcg | Standard community daily draw (12 IU after 3ml BAC). Sized to GHK-Cu. The other two peptides come along at the vial ratio. |
| Week 6 | GHK-Cu 2mg + BPC-157 400mcg + TB-500 400mcg | Last on-week of a 6-week cycle. Stop and assess rather than opening a second vial by default. |
| Weeks 7-10 | Off | Deload. GHK-Cu delivers copper, which is why this blend is cycled instead of run continuously. |
Mixing Math
Reconstitution
Reference values for preparing a research vial. Adjust the syringe reading if you change the water volume.
Vial size
70mg (GHK-Cu 50mg + BPC-157 10mg + TB-500 10mg)
BAC water
3ml bacteriostatic water
Concentration
~23.3mg/ml total (GHK-Cu ~16.7mg/ml)
Dose / ml
2mg GHK-Cu = 0.12ml (delivers 400mcg BPC + 400mcg TB)
Syringe units
12 IU on U-100 for the standard daily draw
Frequently Paired
Common Stacks
Storage
Handling Notes
Lyophilized blend: 2-8°C preferred, -20°C for long storage. Reconstituted: refrigerate at 2-8°C and use within 21-28 days. Protect from light. The solution is often faintly blue from the copper complex; that tint is the GHK-Cu, not contamination. Do not freeze reconstituted solution.
Research Considerations
Cautions
- There is no published human or animal trial of GHK-Cu, BPC-157, and TB-500 given together. Component papers are not blend evidence.
- The 50/10/10 ratio is fixed. You cannot independently raise BPC-157 or TB-500 from this vial. Confirm the printed ratio; some sellers ship a different split under the same GLOW name.
- Clinic 'GLOW' shots made of glutathione and ascorbic acid are a different product. If the label does not name all three peptides, it is not this chart.
- GHK-Cu often stings on injection. Rotate sites and do not reuse the same spot for 48 hours.
- Copper exposure is why 4-6 weeks on and a multi-week off window is the conservative pattern. Do not stack this vial with other daily copper peptides or high-dose topical GHK-Cu for the same stretch.
Primary Sources
Research Behind This Protocol
Maquart 1988 · FEBS Letters
Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+
PubMed 3169264Pickart 2018 · International Journal of Molecular Sciences
Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data
PubMed 29986520Chang 2011 · Journal of Applied Physiology
The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration
PubMed 21030672Philp 2003 · Wound Repair and Regeneration
Thymosin beta 4 and a synthetic peptide containing its actin-binding domain promote dermal wound repair in db/db diabetic mice and in aged mice
PubMed 12581423
Keep researching
Next steps for GLOW Peptide
The chart is the protocol. The stack page, the comparison, and the vendor audit sit one click away.
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GLOW Blend
What each peptide is doing in the vial, how GLOW differs from Wolverine, and when KLOW is the better fourth-peptide add.
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GLOW vs KLOW
Same 50/10/10 base. KLOW adds KPV. Use this when the research question is gut or barrier inflammation, not extra collagen.
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Ascension Peptides review
The 70mg GLOW SKU this chart is built around, plus how we grade their per-lot COAs. Code ENHANCED is 50% off.
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